Using automatic pipettors
You will use precision variable volume micropipettors for liquid handling in every single laboratory session in BIOC 111. If our only purpose was to give you plenty of practice using these devices, which are found in just about every biological research laboratory in just about every discipline, that would be reason enough. Fact is, though, you will need to pipet precise liquid volumes in the microliter range in order to successfully complete any experiment that you conduct not only in this laboratory course but also in other laboratory courses and in research labs. Most meaningful biological research involves mixing precise quantities of cells, DNA, enzymes or other proteins, anitbodies, etc. Too much or too little and your experiment fails. We use liquid handling devices to mix these components because they are almost always dissolved or suspended in liquid, and because it is far easier to transfer a precise volume of liquid than it is to transfer a precise mass of solid material.
Your one and only learning objective regarding automatic pipettors is:
to be able to use an automatic variable volume pipettor to reliably and precisely transfer a selected small volume (between 1 and 1000 microliters) each and every time such transfer is required to complete an experimental protocol
Below are some tips that should help you achieve this objective.
On the first day of lab, you will get a chance to create a colorful design with pipettor pointillism. Make sure you record a detailed key in your lab notebook for your design, including colors, volume, and desired pattern.
We would like to thank New England Biolabs for their generous support of our laboratory program
Copyright and Intended Use
Visitors: to ensure that your message is not mistaken for SPAM, please include the acronym "Bios211" in the subject line of e-mail communications
Created by David R. Caprette (email@example.com), Rice University 14 Jul 08
Author: Beth Beason Abmayr, Ph.D., Rice University
Updated 2 July 2014